plasmid encoding mcherry-sec61β Search Results


pegfp n2 vector  (TaKaRa)
96
TaKaRa pegfp n2 vector

Pegfp N2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding mcherry sec61β  (Addgene inc)
94
Addgene inc plasmid encoding mcherry sec61β

Plasmid Encoding Mcherry Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding mcherry-sec61β  (Addgene inc)
90
Addgene inc plasmid encoding mcherry-sec61β

Plasmid Encoding Mcherry Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids encoding mcherry sec61β  (Addgene inc)
94
Addgene inc plasmids encoding mcherry sec61β

Plasmids Encoding Mcherry Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry rab5  (Addgene inc)
93
Addgene inc mcherry rab5

Mcherry Rab5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry tomm20  (Addgene inc)
93
Addgene inc mcherry tomm20

Mcherry Tomm20, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry sec61β  (Addgene inc)
93
Addgene inc mcherry sec61β

Mcherry Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tom70 mcherry frb  (Addgene inc)
94
Addgene inc tom70 mcherry frb
( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial <t>TOM70-GFP-FRB</t> and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.
Tom70 Mcherry Frb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmturquoise2 golgi  (Addgene inc)
93
Addgene inc pmturquoise2 golgi
( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial <t>TOM70-GFP-FRB</t> and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.
Pmturquoise2 Golgi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptriex ntom20 lov2  (Addgene inc)
92
Addgene inc ptriex ntom20 lov2
( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial <t>TOM70-GFP-FRB</t> and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.
Ptriex Ntom20 Lov2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cib1 mcerulean mp  (Addgene inc)
95
Addgene inc cib1 mcerulean mp
( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial <t>TOM70-GFP-FRB</t> and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.
Cib1 Mcerulean Mp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry erlumen  (Addgene inc)
93
Addgene inc mcherry erlumen
( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial <t>TOM70-GFP-FRB</t> and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.
Mcherry Erlumen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: eLife

Article Title: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

doi: 10.7554/eLife.65192

Figure Lengend Snippet:

Article Snippet: Plasmid encoding human S1R gene fused with GFP (S1R-GFP) was generated by PCR amplification of the human S1R gene ( http://www.ncbi.nlm.nih.gov/nuccore/NM_005866.3 ) and cloning into pEGFP-N2 vector (Clontech) using HindIII/XbaI cloning sites. mCherry-Sec61β was obtained from Addgene ( https://www.addgene.org/49155 ) ( ).

Techniques: Generated, Selection, Isolation, Recombinant, Plasmid Preparation, Mutagenesis, Construct, Clone Assay, Sequencing, Software

( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial TOM70-GFP-FRB and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.

Journal: bioRxiv

Article Title: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

doi: 10.1101/544700

Figure Lengend Snippet: ( A ) Schematic of RNb-FKBP bound to RFP. ( B ) Schematic of GNb-FKBP bound to GFP. ( C, D ) HeLa cells co-expressing RNb-FKBP, mitochondrial TOM70-GFP-FRB and mCh-Sec61β were imaged using TIRFM. A representative cell (n = 7) is shown before (C) and after (D) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( E ) Timecourse of mCh-Sec61β fluorescence changes (F/F 0 ) evoked by rapamycin recorded at a representative mitochondrion and in nearby reticular ER. Results show ~80% loss of fluorescence from the ER devoid of mitochondrial contacts. ( F , G ) HeLa cells co-expressing endogenously tagged GFP-IP 3 R1, GNb-FKBP and mitochondrial TOM70-mCh-FRB were imaged using TIRFM. A representative cell (n = 6) is shown before (F) and after (G) treatment with rapamycin (100 nM, 10 min). The boxed region is enlarged in subsequent images. Scale bars 10 µm (main images) and 2.5 μm (enlargements). ( H ) HeLa cells co-expressing GFP-calmodulin (GFP-CaM), GNb-FKBP and TOM20-mCh-FRB were imaged using epifluorescence microscopy. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 nM, 10 min). The image for TOM-mCh-FRB is shown in the presence of rapamycin. Scale bar 10 μm.

Article Snippet: Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEM-CEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

Techniques: Expressing, Fluorescence, Epifluorescence Microscopy

( A ) Schematic of RNb-FKBP fusion bound to RFP. ( B, C ) HeLa cells co-expressing RNb-FKBP, mitochondrial TOM70-GFP-FRB and β 2 AR-mCh were imaged using TIRFM before (B) and after (C) treatment with rapamycin (100 nM, 10 min). Scale bar 10 µm. ( D, E ) Enlarged images from C of the yellow box (D) and cyan box (E) show punctate recruitment of β 2 AR-mCh to individual mitochondria at the indicated times after addition of rapamycin. Scale bars 1.25 µm. ( F ) TIRFM images of HeLa cells co-expressing mitochondrial TOM70-GFP-FRB and β 2 AR-mCh in the presence of rapamycin (100 nM, 10 min) show no recruitment in the absence of co-expressed RNb-FKBP. The yellow box shows a region enlarged in the subsequent image. Scale bars 10 µm (main images) and 2.5 μm (enlargement). Results (B-F) are representative of 5 independent experiments.

Journal: bioRxiv

Article Title: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

doi: 10.1101/544700

Figure Lengend Snippet: ( A ) Schematic of RNb-FKBP fusion bound to RFP. ( B, C ) HeLa cells co-expressing RNb-FKBP, mitochondrial TOM70-GFP-FRB and β 2 AR-mCh were imaged using TIRFM before (B) and after (C) treatment with rapamycin (100 nM, 10 min). Scale bar 10 µm. ( D, E ) Enlarged images from C of the yellow box (D) and cyan box (E) show punctate recruitment of β 2 AR-mCh to individual mitochondria at the indicated times after addition of rapamycin. Scale bars 1.25 µm. ( F ) TIRFM images of HeLa cells co-expressing mitochondrial TOM70-GFP-FRB and β 2 AR-mCh in the presence of rapamycin (100 nM, 10 min) show no recruitment in the absence of co-expressed RNb-FKBP. The yellow box shows a region enlarged in the subsequent image. Scale bars 10 µm (main images) and 2.5 μm (enlargement). Results (B-F) are representative of 5 independent experiments.

Article Snippet: Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEM-CEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

Techniques: Expressing

( A ) Schematic of GNb-FKBP fusion bound to GFP. ( B ) HeLa cells co-expressing mitochondrial TOM70-mCh-FRB (magenta), lysosomal LAMP1-GFP (green) and GNb-FKBP were imaged using TIRFM. Merged images of a representative cell (n = 5) are shown before and at times after treatment with rapamycin (rapa, 100 nM). Scale bar 10 µm. ( C ) Enlargements of the boxed region in (B). Scale bar 2.5 μm. ( D ) HeLa cells co-expressing TOM70-mCh-FRB (magenta) and lysosomal LAMP1-GFP (green) were imaged using TIRFM. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 µm, 10 min); there is no recruitment in the absence of co-expressed GNb-FKBP. The yellow box shows a region enlarged in the subsequent image. Scale bars 10 µm (main images) and 2.5 μm (enlargement).

Journal: bioRxiv

Article Title: A genetically-encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling

doi: 10.1101/544700

Figure Lengend Snippet: ( A ) Schematic of GNb-FKBP fusion bound to GFP. ( B ) HeLa cells co-expressing mitochondrial TOM70-mCh-FRB (magenta), lysosomal LAMP1-GFP (green) and GNb-FKBP were imaged using TIRFM. Merged images of a representative cell (n = 5) are shown before and at times after treatment with rapamycin (rapa, 100 nM). Scale bar 10 µm. ( C ) Enlargements of the boxed region in (B). Scale bar 2.5 μm. ( D ) HeLa cells co-expressing TOM70-mCh-FRB (magenta) and lysosomal LAMP1-GFP (green) were imaged using TIRFM. A representative cell (n = 3) is shown before and after treatment with rapamycin (100 µm, 10 min); there is no recruitment in the absence of co-expressed GNb-FKBP. The yellow box shows a region enlarged in the subsequent image. Scale bars 10 µm (main images) and 2.5 μm (enlargement).

Article Snippet: Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1); GFP-ERcyt, mCherry-ERcyt and mTurquoise2-ERcyt (GFP, mCherry or mTurquoise2 targeted to the cytosolic side of the ER membrane via the ER-targeting sequence of the yeast UBC6 protein) [ ]; mCherry-ERlumen (Addgene #55041, provided by Michael Davidson); LAMP1-mCherry [ ]; TPC2-mRFP [ ]; TOM20-mCherry (Addgene #55146, provided by Michael Davidson); CIB1-mRFP-MP (Addgene #58367) [ ]; CIB1-mCerulean-MP (Addgene #58366) [ ]; H2B-GFP (Addgene #11680) [ ]; TOM20-LOV2 (Addgene #81009) [ ]; mCherry-Sec61β [ ]; GFP-MAPPER [ ]; GFP-CaM (Addgene #47602, provided by Emanuel Strehler); TOM70-mCherry-FRB (pMito-mCherry-FRB, Addgene #59352) [ ]; pmTurquoise2-Golgi (Addgene #36205) [ ]; pTriEx-mCherry-zdk1 (Addgene #81057) [ ]; pTriEx-NTOM20-LOV2 (Addgene #81009) [ ]; β 2 AR-mCFP (Addgene #38260) [ ]; pCMV-G-CEPIA1er (Addgene #58215) [ ]; pCMV-R-CEPIA1er (Addgene #58216) [ ]; pCIS-GEM-CEPIA1er (Addgene #58217) [ ]; CMV-ER-LAR-GECO1 and CMV-mito-LAR-GECO1.2 [ ]; mCherry-MAPPER and mCherry-Orai1 [ ].

Techniques: Expressing